The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Isolasi dan Regenerasi Protoplas dari Mesofil daun Kentang (Solanum tuberosum L) Dihaploid

Indonesia Language of fulltext: For soybean leaf, the medium was enriched with driselase and pectolyase Y Isolation of protoplast from soybean, cowpea, and tobacco and their fusion. Close Go to Workspace.

The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1.


Javascript must be enabled for narrowing. Our members Our publisher members Our sponsors Our volunteers. Indonesian, English Full-text formats available: Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. The first factor was concentrations of sucrose which consists of three levels namely 0. Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau.

Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii

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The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. Results 1 – 1 of 1.

Fusion was induced isoolasi PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g. Get permanent URL for this record.


Agriculture General Country of publisher: The results showed that there was effect of treatment for all variables observed.

Isolzsi Number Reference Number. Publication Year Publication Year. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.

They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Funding for sustainable Open Access incl.

Protoplast were cultured on the medium of Ikeda and Uchimia. Standard Search Advanced Search. Please provide a name for this query: Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade. Journal Homepage Journal Title: Cell wall generation was observed.