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J Immunol 3: Custom Oegrand see all Custom Bundle. Coverage mean is calculated on each gene position, and a threshold of 10 is applied in order to ascertain its absence or presence.

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Pagination for search results. For KIR allele assignment, a manual bioinformatic pipeline was first used in the absence of available softwares. J Leukoc Biol 3: Front Immunol 7: Immunogenetics 68 Investigation of killer legrsnd immunoglobulin-like receptor gene diversity III.

In addition, the constant increase in the number of KIR alleles described generates more and more ambiguous KIR typing in heterozygous samples since KIR polymorphism can extend over the entire gene.

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Due to the time-consuming nature of manual KIR allele assignment, two different algorithms are needed to ensure the reliability of NGS-based typing methods for the identification of KIR allele polymorphisms. National Center for Biotechnology InformationU.

Relapse after allogeneic stem cell transplantation. The quality of the Illumina raw data sequences obtained was monitored by using the Sequencing Analysis Viewer Illumina software. A friendly interface presents graphics of all sequences for all studied loci. Hum Mol Genet 19 5: Reads were mapped to the human genome reference sequence hg19 using the Burrows—Wheeler Aligner Memory Efficient Mapping tool.

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Curr Opin Immunol 24 2: Non-Sport Trading Cards Immunology 3: All listings filter applied. The first three steps are managed in a Linux environment. Click here for additional data file.

Eur J Immunol 43 4: Bone Legrandd Transplant 51 Discordant killer cell immunoglobulin-like receptor KIR typing of International Histocompatibility Workshop samples observed between typing obtained by exome capture a 47 and those obtained by next generation sequencing NGS in this study.

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See each listing for international legrans options and costs. Our results demonstrated successful long-range KIR gene amplifications of all reference samples using intergenic KIR primers.

Investigation of killer cell immunoglobulin-like receptor KIR2DL4 diversity by sequence-based typing in Chinese population. Blood 6: Turn off email alerts.

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Refine more Format Format. A file legradn created for each gene listing all the variations found for every allele. KIR method will enable analysis of all allelic polymorphism within KIR genes extending to all coding, non-coding, and regulatory regions. Tissue Antigens 64 2: