Horizon Scanning Technology. Prioritising Summary. LifePort. ® kidney transporter: A portable donor kidney transporter/ perfuser. November 24 – What to do after pumping begins. 28 – Removing a kidney from LifePort Kidney Transporter; removing used Perfusion Circuit after a case. 34 – 45 . The LifePort Kidney Transporter is a revolutionary method of transporting kidneys for transplantation: it is a portable, insulated perfusion transporter with.
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To overcome the shortage of brain-dead donors for liver transplantation, extended criteria donors ECDs have been used 1. An electronic scale was installed under the liver container to record the increase of weight per min.
Protective effects of a carbon monoxide-releasing molecule CORM-3 during hepatic cold preservation. Nowadays, it has been demonstrated that cold storage, a traditional organ preservation technique, cannot meet the demands of ECD preservation due to the reduced ischemic tolerance of these marginal grafts 78.
By contrast, according to the preliminary results in the present study, the prime mode of Lifeport could achieve continuous perfusion, which was indicated by the oscillogram with a high frequency and small wave range. When creating this portable device it was vital that the extensive engineering complexities were solved, and it had to be easily transported by a wide variety of hospital personnel and air and other transport personnel.
An electronic scale was installed under the liver container to calculate the portal inflow according to the association with weight, density and volume of the perfusate.
The Lifeport Kidney Transporter
Bile was collected from the tube placed in the common bile duct at the end of HMP. An intravenous injection of 2 ml saline with U heparin Nanjing Jiancheng Bioengineering Institute, Nanjing, China via the right iliac vein was performed for heparinization Liver samples were analyzed using hematoxylin and eosin staining Fig.
B Overall appearance of the modified HMP system. Outcome of liver transplantation using donors older than 60 years of age.
The Lifeport Kidney Transporter – INDEX: Design to Improve Life®
ALT 0 h, Fluorescent dyes for cell viability: Extended criteria donors in liver transplantation Part I: Reviewing the impact of determining factors. H; Nanjing Jiancheng Bioengineering Institute in accordance with the manufacturer’s protocol. IR was calculated according to the following formula: Detection of endothelin was performed to determine the level of shear stress and pressure damage to the endothelial cell layer due to HMP. Assessment of islet cell viability using lifdport dyes.
HMP may immediately terminate once the perfusion pressure exceeds the upper limit of the LifePort. Therefore, the operator was required to monitor the change of pressure prior to IR stabilization, particularly at the beginning of HMP. The isolated perfused rat liver: Role of oxygen during hypothermic machine perfusion preservation of the liver. China Find articles by Xianpeng Zeng.
Continuous HMP via the portal vein for liver preservation is capable of simulating hemodynamics of the portal vein and has been widely applied in a number of previous studies 1221 C and lactate dehydrogenase assay kit cat no. The images were analyzed using the point-counting method within grids that contained 80 points combined with ImageJ 1.
Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats. Data management and sample collection The flow, pressure, intrahepatic resistance IRand temperature were recorded every 3 h for a total of 6 h during HMP.
C The modified system installed on the LifePort. Abstract The protective mechanisms for liver preservation associated with hypothermic machine perfusion HMP remain unclear. OC, ATP production and bile production were markers to assess cellular metabolic activity. China Find articles by Xiaoyan Hu.
The dynamics inflow, pressure and intrahepatic resistance of perfusion were assessed to identify whether this system met the demands for HMP of rat livers. The LifePort Kidney Transporter preserves and protects a donated kidney during the sometimes long, cold journey from recovery to transplantation.
These findings were in accordance with those observed previously in similar recirculating perfusion devices 1225 and indicated the shear stress and pressure injury of the present HMP system was minimal, and that the increased ALT and LDH release was likely due to cold lifepkrt injury.